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Quantitative Western Blot

Western blotting can be used to monitor protein levels, post-translational modifications, and to understand the mechanism of action for drug candidates. Protein abundance is evaluated by in lane normalization to a housekeeping protein, when possible.

Figure 1: Detection and analysis of Tau protein in rTg4510, a mouse model of tauopathy, by quantitative western blotting. Tau protein was most abundant in rTg4510 mouse brains at 6 months of age as compared to that of age-matched tTA control mice. Tau detection was carried out with ECL Plus chemifluorescence (FAM) and GAPDH, a housekeeping protein, was detected by direct fluores cence with rhodamine-conjugated GAPDH antibody.

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PsychoGenics Inc.
215 College Road, Paramus, NJ 07652

Email: info@psychogenics.com
Phone: (914) 406-8019

Fax: (914) 406-8090

 

Copyright © 2020 PsychoGenics Inc.

All rights reserved.

CONTACT

PsychoGenics Inc.
215 College Road, Paramus, NJ 07652

Email: info@psychogenics.com
Phone: (914) 406-8019

Fax: (914) 406-8090

Copyright © 2020 PsychoGenics Inc.

All rights reserved.