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Quantification of Phosphoproteins and Secondary Messengers

On-target effects of compounds can be evaluated by quantification of brain phosphoproteins and secondary messengers. PsychoGenics has set up and validated brain focused microwave irradiation technology for maximal preservation of phosphorylated proteins and secondary messengers in vivo.

Figure 1: PDE10 Inhibition increases CREB Phosporylation. Stimulation of phosphorylation of CREB (Ser133) by the PDE10 inhibitor TP-10. Mice were treated subcutaneously with either vehicle (40% Beta-cyclodextrin) or TP-10 (3.2 mg/kg) for 30 minutes and were then sacrificed by focused microwave irradiation. Striatal pCREB levels were analyzed with Western blot (Top Panel) (each lane corresponds to a single animal) or ELISA (Bottom Panel).

CONTACT

PsychoGenics Inc.
215 College Road, Paramus, NJ 07652

Email: info@psychogenics.com
Phone: (914) 406-8019

Fax: (914) 406-8090

 

Copyright © 2020 PsychoGenics Inc.

All rights reserved.

CONTACT

PsychoGenics Inc.
215 College Road, Paramus, NJ 07652

Email: info@psychogenics.com
Phone: (914) 406-8019

Fax: (914) 406-8090

Copyright © 2020 PsychoGenics Inc.

All rights reserved.