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S. OAKESHOTT, J. SHAMY, A. PAINTDAHKI, R. PORT, D. VELEDNITSKY, I. FILIPPOV, C. PATER, M. PATRY, L. MENALLED, C. MURPHY, V. ALEXANDROV, S. RAMBOZ, J. WATSON-JOHNSON, M. RUIZ, D. LAROSE, J.-S. VALOIS, D. HOWLAND, S. KWAK, D. BRUNNER

Mice were longitudinally tested in a novel automated phenotyping system based on Intellicage (©New Behavior AG) with substantial in-house modifications to allow automated video scoring. This system is able to simultaneously monitor cognitive, social, circadian and motor behaviors across the animals’ daily activity cycle, without the need to handle or interfere with the mice in any way. We tested R6/2 transgenic mice, a widely used genetic model of Huntington’s disease (Mangiarini et al., 1996), in two studies designed to evaluate progression of the disease phenotype with age. In the first experiment, mice were tested at 7-wks, 12-wks and 14-wks of age, while in the second animals were tested at 7-wks, 8-wks, 9-wks and 10-wks of age. In both studies, the R6/2 TG animals displayed a progressive and diverse phenotype, with deficits measured across several of the behavioral domains recorded by the system. These data are comparable to results obtained with more traditional individual tests of each of the relevant behaviors, suggesting that this system might provide a high-throughput means to quickly screen and characterize different rodent disease models.