The Morris water maze test assesses hippocampal-dependent spatial learning and memory. It consists of a water pool with a hidden escape platform and using visual cues, rodents learn over the course of days to find the hidden platform and escape from the water.

Following training for 4 - 6 days mice were given a probe trial where the platform is removed. Memory index was measured by quantifying the time spent in the correct quadrant during the probe test. Pretreatment with physostigmine increased the memory index of the mice.